王华，任小娜，许新兵，陈维铅，许世鹏

（甘肃省太阳能发电系统工程重点实验室 酒泉职业技术学院，甘肃 酒泉 735000）

［摘 要］采用超声波辅助草取法提取，高效液相色请法测定了内器（Cinhe）中的儿茶素含量。在单因素试验基础上，以料液比、提取温度、超声时间、提取次数为研究对象，进行了4因素3水平正交试验。结果表明内欢蓉中儿茶素最佳提取工艺为。甲醇料液比2g：25ml，提取温度60℃，超声提取30min，提取1次。儿茶素在0.602-15.05 pghL．范围内呈良好线性关系，回归方程为y-19300x＋421095.1-0.9996．加样回收率为98.25％。在条件下，肉成蓉中儿茶素含量为25.77mg／g。

［关键词］高效液相色谱：内苁蓉：儿茶素：提取：含量测定       

 ［文章编号］1007-1865（2020）05-0196-03

［中分类号］0657.7＋2          ［文献标识码］A

Study on Extraction Conditions and Content Determination of Catechin from

Cistanche

Wang Hua,Ren Xiaona,Xu Xinbing,Cheng Weiqian,Xu Shipeng

(Gansu Key Laboratory of Solar Power Generation System Project,Jiuquan Vecational Technical College,Jiuquan 735000,China)

Abstractt Catechin in Citanche was alrasonically extracted and detectod by HPLC.Acconding o single-factor experiments,three level of four factors incliting material -lkguid ratio,uhravonic emperature,alrasonic tme and ulrasonic mumber were sleced for the orthogonal design.The resuls showed that the optimum extraction condtions as follows:makrialiqud ratio was2g:25ml nih 30 mn ulrasonicansiod extaction under 60 C,with anlyone ultrasound was performed.Uinder the aptimal conditions,the cahbration cure of Chiectin was linca(-0.9996)in the range of 0.502-15.05 ughl,The equation was y-19300x+421095,-0.9998,the recovery was 98.26 %and the conent of Catectin from Catnche was 26.77 mg/g This process can effectively extrat catechin from cistanche,and provide a bavis for the improvement of pharmacopocia sandard in the fture.

Keywordst high performance lipud chromaograply:Caunche Catechin estactions desermination

肉苁蓉（Catanche deserticola YC.Ma）是我国中医用途最广的珍贵中草药之一，习称大芸、苁蓉、为列当科（Oobanchancae）肉苁蓉属（Cistanche）多年生寄生草本植物，专性寄生在藜科（Chenopodiaceae）校梭（Haloncylon ammodendon（C.A.Mey）Bunge） 和白梭梭（H.Persicum Bungeex Boss）的根部。主产于我国内蒙、新疆、甘肃、宁夏、青海等省（区）以及伊朗、蒙古、印度等国。其味甘咸微辛酸、微温。在《神农本草经》中将其列为上品，中国药真规定正品为干燥带鳞叶的肉质。肉苁蓉具有强肝肾。益精血，滑肠通便之功效，动物实验显示有降压作用，其中所含儿茶素具有提高免疫功能、清除自由基、抗氧化作用、防突变。抗癌等活性，与肉苁蓉主要药理作用相吻合。本课题经 HPLC预试验发现儿茶素含量较高，但中国药典对其含量的测定方法却没有具体标准，面对肉苁蓉中儿茶素的含量测定目前尚未见有文献报道。因此，本文将对肉苁蓉中儿茶素的提取及含量测定进行条件优化及探索，希望找到一条切实可行的测定方法，为今后肉苁蓉的进一步研究开发提供依据。